P. Narayanasamy – Plant Pathogen Detection and Disease Diagnosis (2nd edition)

Автор: P. Narayanasamy
Название книги: Plant Pathogen Detection and Disease Diagnosis (2nd edition)
Формат: PDF
Жанр: Биологические науки
Страницы: 533
Качество: Изначально компьютерное, E-book

In this book, both conventional and modern molecular methods of detecting plant pathogens have been described in an easily understandable manner to enable researchers both in the laboratory and the field, who may not be very familiar with molecular techniques, to understand and follow these methods in their investigations. Many examples for the detection, identification, differentiation, and quantification of different kinds of microbial pathogens are included, with protocols in appendices of the appropriate chapters. The usefulness and limitations of the different methods are indicated. Researchers and personnel of disease diagnostic centers, plant protection, plant quarantine, and seed certification services will find a wide choice of techniques to suit the requirements and facilities available. It is hoped that this book will serve as a valuable reference in this aspect of plant pathology.
Contents.
Foreword (M.S. Swaminathan).
Preface to the Second Edition.
Preface to the First Edition.
Introduction.
Nature and Causes of Plant Diseases.
Disease Diagnosis and Management Strategies.
Characteristics of Pathogenic Microbes.
Light Microscopy.
Methods of Isolation of Pathogens.
Fungi.
Bacteria.
Phytoplasmas.
Viruses.
Viroids.
Appendix.
Symptoms of Plant Diseases.
External Symptoms.
Cytopathological Characteristics.
Molecular Genetics of Pathogenicity.
Appendix.
Dissemination of Plant Pathogens.
Transmission of Plant Viruses.
Transmission of Viroids.
Transmission of Bacterial Pathogens.
Transmission of Fungal Pathogens.
Cross-Protection.
Chemodiagnostic Methods.
Detection of Plant Viruses.
Detection of Phytoplasmas.
Detection of Fungal Pathogens.
Detection of Bacterial Pathogens.
Detection of Viroids.
Appendix.
Electron Microscopy.
Preparation of Support Films.
Calibration of the Electron Microscope.
Techniques for Identification of Plant Viruses.
Techniques for Detection of Phytoplasmas.
Techniques for Detection of Fungal Pathogens.
Techniques for Detection of Bacterial Pathogens.
Scanning Electron Microscopy.
Appendix.
Serodiagnostic Methods.
Detection of Plant Viruses.
Detection of Phytoplasmas.
Detection of Plant Pathogenic Bacteria.
Detection of Fungal Pathogens.
Detection of Viroids.
Appendix.
Nucleic Acid–Based Techniques.
Hybridization Methods.
Detection of Plant Viruses.
Detection of Viroids.
Polymerase Chain Reaction.
Detection of Phytoplasmas.
Detection of Fungal Pathogens.
Detection of Bacterial Pathogens.
Appendix.
Detection of Double-Stranded RNAs.
Diagnosis and Monitoring of Plant Diseases.
Plant Disease Diagnostic Centers.
Plant Quarantines.
References.