F. Christiansen – Immunogenetics

Автор: F. Christiansen
Название книги: Immunogenetics
Формат: PDF
Жанр: Биологические науки
Страницы: 690
Качество: Изначально компьютерное, E-book

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.
Contents
The Major Hisompatibility Complex – A Paradigm for Studies of the Human Genome
HLA Typing by SSO and SSP Methods
Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity
HLA Typing Using Bead Based Methods
HLA Typing by Direct DNA Sequencing
Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio
Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions
Molecular Typing of HLA-E
Molecular Analysis of Complement Component C4 Gene Copy Number
Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5’ Nuclease Allelic Discrimination
High Resolution MICA Genotyping by Sequence-Based Typing (SBT)
Standard Methods for the Management of Immunogenetic Data
Analytical Methods for Immunogenetic Population Data
Analytical Methods for Disease Association Studies with Immunogenetic Data Jill A. Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View
Screening for Antibodies Against MICA by Luminex Flow Cytometry
HLA Antibody Detection and Characterization by Solid Phase Immunoassays – Methods and Pitfalls
Detection and Characterisation of Alloreactive T Cells
Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells
Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node
Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC)
The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation
Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System
KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP)
Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing
An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation
The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay
Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells
Minor Hisompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation
Donor Registries and Search Strategies
Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance
IMGT® Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS
IMGT/DomainGapAlign: the IMGT® Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism
Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism