M. Bolton — Plant Fungal Pathogens

Автор: M. Bolton
Название книги: Plant Fungal Pathogens
Формат: PDF
Жанр: Биологические науки
Страницы: 665
Качество: Изначально компьютерное, E-book

This book brings together over 40 chapters that contribute toward our understanding of the biology and pathology of fungal plant pathogens. Drawing on techniques utilizing model systems such as Arabidopsis thaliana as well as agricultural crop plants, this book highlights some of the latest techniques critical for students pursuing molecular plant pathology. However, most protocols can be adapted to any pathosystem or fungal pathogen. Since pathogenicity and disease development may depend on effector proteins secreted by plant pathogens during infection, several chapters are dedicated to various means of identifying effectors. In addition, protocols on various aspects of fungal biology are included, including those utilizing molecular biology, proteomics, and metabolomics. Given the recent deluge of fungal genome sequencing projects, chapters on genome annotation and next-generation sequencing of fungal genomes are included. Finally, protocols for gene knock-outs, fungal transformation, and molecular tools for disease and/or pathogen quantification are included that are critical for revealing the role for a fungal gene of interest in disease development.
Taken together, the protocols and review chapters in this book are timely and provide a current set of techniques that cover a wide-range of methods to study molecular aspects of pathogenesis. It is hoped that this book will be useful for those new to the field of molecular plant pathology as well as experienced fungal research laboratories.
Contents:
Fungal ABC Transporter Deletion and Localization Analysis (Andriy Kovalchuk, Stefan S. Weber, Jeroen G. Nijland, Roel A.L. Bovenberg, and Arnold J.M. Driessen).
Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens-Mediated Transformation (Rasmus John Normand Frandsen, Mette Frandsen, and Henriette Giese).
Activity-Based Protein Profiling of Infected Plants (Farnusch Kaschani, Christian Gu, and Renier A.L. van der Hoorn).
The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves (Lisong Ma, Ewa Lukasik, Fleur Gawehns, and Frank L.W. Takken).
Proteomic Techniques for Plant–Fungal Interactions (Delphine Vincent, Kar-Chun Tan, Liam Cassidy, Peter S. Solomon, and Richard P. Oliver).
Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway-Compatible Binary Potato Virus X-Expression Vector (H. Peter van Esse).
Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens (Jayma A. Moore and Scott A. Payne).
Real-Time PCR for the Quantification of Fungi In Planta (Steven J. Klosterman).
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi (Mark Arentshorst, Arthur F.J. Ram, and Vera Meyer).
Atomic Force Microscopy: A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates (Elizabeth Adams, Danielle Emerson, Sean Croker, Hye-Seon Kim, Shannon Modla, Seogchan Kang, and Kirk Czymmek).
Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors (Shunwen Lu).
Characterization of Plant-Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens (Timothy L. Friesen and Justin D. Faris).
Heterologous Production of Fungal Effectors in Pichia pastoris (Anja Kombrink).
The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta (Wei-Hua Tang, Yan Zhang, and Jon Duvick).
Metabolomics Protocols for Filamentous Fungi (Joel P.A. Gummer, Christian Krill, Lauren Du Fall,
Ormonde D.C. Waters, Robert D. Trengove, Richard P. Oliver, and Peter S. Solomon).
Targeted Gene Replacement in Fungi Using a Split-Marker Approach (Rubella S. Goswami).
Massively Parallel Sequencing Technology in Pathogenic Microbes (Sucheta Tripathy and Rays H.Y. Jiang).
Confocal Microscopy in Plant–Pathogen Interactions (Adrienne R. Hardham).
The Use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data (Benjamin M. Nitsche, Arthur F.J. Ram, and Vera Meyer).
Population Biology of Fungal Plant Pathogens (Zahi K. Atallah and Krishna V. Subbarao).
Polyethylene Glycol (PEG)-Mediated Transformation in Filamentous Fungal Pathogens (Zhaohui Liu and Timothy L. Friesen).
In Vitro Induction of Infection-Related Hyphal Structures in Plant Pathogenic Fungi (W.R. Rittenour and S.D. Harris).
Fungicide Resistance Assays for Fungal Plant Pathogens (Gary A. Secor and Viviana V. Rivera).
Identification of Lipid-Binding Effectors (Shiv D. Kale and Brett M. Tyler).
In Silico Identification and Characterization of Effector Catalogs (Ronnie de Jonge).
Horizontal Transfer of Supernumerary Chromosomes in Fungi (H. Charlotte van der Does and Martijn Rep).
The Induction of Mycotoxins by Trichothecene Producing Fusarium Species (Rohan Lowe, Mélanie Jubault, Gail Canning, Martin Urban, and Kim E. Hammond-Kosack).
Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics (J.B. Ridenour, R.L. Hirsch, and B.H. Bluhm).
Assessment of Autophagosome Formation by Transmission Electron Microscopy (Marina Nadal and Scott E. Gold).
Fungal Plant Pathogen Detection in Plant and Soil Samples Using DNA Macroarrays (B. Lievens, A. Justé, and K.A. Willems).
Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors (Parthasarathy Santhanam).
A Yeast Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and Their Plant Hosts (Sang-Jik Lee and Jocelyn K.C. Rose).
Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes (Eva H. Stukenbrock and Julien Y. Dutheil).
Multigene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens (Tara L. Rintoul, Quinn A. Eggertson, and C. André Lévesque).
MAP Kinase Phosphorylation and cAMP Assessment in Fungi (Rahim Mehrabi, Sarrah Ben M’Barek, Abbas Saidi, Masoud Abrinbana, Pierre J.G.M. de Wit, and Gert H.J. Kema).
A One-Step Affinity-Purification Protocol to Purify NB-LRR Immune Receptors from Plants That Mediate Resistance to Fungal Pathogens (Wladimir I.L. Tameling).
Karyotyping Methods for Fungi (Rahim Mehrabi, Masatoki Taga, Mostafa Aghaee, Pierre J.G.M. de Wit, and Gert H.J. Kema).
Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique (Matthieu H.A.J. Joosten).
Gene Cloning Using Degenerate Primers and Genome Walking (Javier A. Delgado, Steven Meinhardt, Samuel G. Markell, and Rubella S. Goswami).
Construction of Hairpin RNA-Expressing Vectors for RNA-Mediated Gene Silencing in Fungi (Shaobin Zhong, Yueqiang Leng, and Melvin D. Bolton).
An Unbiased Method for the Quantitation of Disease Phenotypes Using a Custom-Built Macro Plugin for the Program ImageJ (Ahmed Abd-El-Haliem).