Marianne Bronner-Fraser – Avian Embryology

Автор: Marianne Bronner-Fraser
Название книги: Avian Embryology (2008)
Формат: PDF
Жанр: Биологические науки
Страницы: 426
Качество: Изначально компьютерное, E-book

This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques.
Embryological Microsurgery and Tissue Culture Methods:
Operations on Primitive Streak Stage Avian Embryos
Quail–Chick Transplantations
Other Chimeras: Quail–Duck and Mouse–Chick
Manipulations of Neural Crest Cells or Their Migratory Pathways
Embryo Slices and Strips: Guidance and Adhesion Assays in the Avian Embryo
Neural Crest, Sensory Neuron, and Muscle Cultures
Methods in Avian Embryology Experimental and Molecular Manipulation of the Embryonic Chick Limb
Cell Division, DiVerentiation, and Death in Avian Embryos
Labeling and Transgenesis Approaches;
In Situ Hybridization Analysis of Chick Embryos in Whole-Mount and Tissue Sections
Vital Labeling of Embryonic Cells Using Fluorescent Dyes and Proteins
Time-Lapse Imaging of the Early Avian Embryo
Gain- and Loss-of-Function Approaches in the Chick Embryo
Manipulation and Electroporation of the Avian Segmental Plate and Somites In Vitro
Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos
Generating Transgenic Quail using Lentiviruses
Functional Genomics:
Gene Discovery: Macroarrays and Microarrays
Dissection of Chick Genomic Regulatory Regions
Computational Approaches to Finding and Analyzing cis-Regulatory Elements
Investigating Regulatory Factors and Their DNA Binding AYnities Through Real Time Quantitative PCR (RT-QPCR) and Chromatin Immunoprecipitation (ChIP) Assays