T. Liehr — Fluorescence In Situ Hybridization (2017)

Автор: T. Liehr
Название книги: Fluorescence In Situ Hybridization (FISH)
Формат: PDF
Жанр: Биологические науки
Страницы: 588
Качество: Изначально компьютерное, E-book

Manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection.
This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.
Contents
Required Equipment and Probes for Fish Procedures
Fish Procedure
Material Suited for Fish Applications in Humans
Multicolor -Fish-Probe Sets (Mfish) and Immunostaining
Interphase Fish
Applications of Fish in Zoology, Botany and Microbiology
Comparative Genomic Hybridization (CGH) and Array CGH