V. Weissig – Liposomes (2 Volume set, 2010)

Автор: V. Weissig
Название книги: Liposomes (2 Volume set)
Формат: PDF
Жанр: Биологические науки
Страницы: 1102
Качество: Изначально компьютерное, E-book

1. Volume 1: Pharmaceutical Nanocarriers
Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving into the depths of the emerging field of pharmaceutical and biomedical nanotechnology. As a volume in the highly successful Methods in Molecular Biology series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Contents
Preface
Contributors
Current Trends in Liposome Research
Nanoliposomes: Preparation and Analysis
Preparation of DRV Liposomes
Elastic Liposomes for Topical and Transdermal Drug Delivery Heather
Archaebacterial Tetraetherlipid Liposomes
Cationic Magnetoliposomes
Ultrasound-Responsive Liposomes
Liposome Formulations of Hydrophobic Drugs
Remote Loading of Antbracyclines into Liposomes
Arsonoliposomes: Preparation and Physicochemical Characterization
Liposome-Based Vaccines
Mannosylated Liposomes for Targeted Vaccines Delivery
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
Vesicular Phospholipid Gels
Environment-Responsive Multifunctional Liposomes
Functional Liposomal Membranes for Triggered Release
A “Dock and Lock” Approach to Preparation of Targeted Liposomes
Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry
Targeted Magnetic Liposomes Loaded with Doxorubicin
Liposomes for Drug Delivery to Mitochondria
Cytoskeletal-Antigen Specific Immunoliposomes: Preservation of Myocardial Viability
Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes
Angiogenic Vessel-Targeting DDS by Liposomalized Oligopeptides
TAT-Peptide Modified Liposomes: Preparation, Characterization, and Cellular Interaction
ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia
Intracellular ATP Delivery Using Highly Fusogenic Liposomes
Lipoplex Formation Using Liposomes Prepared by Ethanol Injection
Acid-Labile Liposome/pDNA Complexes
Serum-Resistant Lipoplexes in the Presence of Asialofetuin
Anionic pH Sensitive Lipoplexes
Liposomal siRNA Delivery
Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparation
Effective In Vitro and In Vivo Gene Delivery by the Combination of Liposomal Bubbles (Bubble Liposomes) and Ultrasound Exposure
Liposomal Magnetofection
Long-Circulating, pH-Sensitive Liposomes
Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes

2. Volume 2: Biological Membrane Models
With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 2: Biological Membrane Models focuses on detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.
Contents
Preface
Contributors
Utilization of Liposomes for Studying Drug Transfer and Uptake
The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles
Use of Liposomes to Study Cellular Osmosensors
Studying Mechanosensitive Ion Channels Using Liposomes
Studying Amino Acid Transport Using Liposomes
Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes
Liposomal Reconstitution of Monotopic Integral Membrane Proteins
The Reconstitution of Actin Polymerization on Liposomes
Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions
Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles
Biosynthesis of Proteins Inside Liposomes
Study of Respiratory Cytochromes in Liposomes
Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity
Studying Colloidal Aggregation Using Liposomes
Assessment of Liposome-Cell Interactions
Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells
The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes
Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling
Membrane Translocation Assayed by Fluorescence Spectroscopy
Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance Spectroscopy
Environmental Scanning Electron Microscope Imaging of Vesicle Systems
Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Atomic Force Microscopy for the Characterization of Proteoliposomes
Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID
Viscometric Analysis of DNA-Lipid Complexes
Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Fluorescence Resonance Energy Transfer-Based Analysis of Lipoplexes
Analysis of Lipoplex Structure and Lipid Phase Changes
Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery
FRET Imaging of Cells Transfected with siRNA/Liposome Complexes
Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes
Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Pre-formed Liposomes for Diagnostic Imaging and Radionuclide Therapy
Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar Vesicles
Liposome Biodistribution via Europium Complexes
Biosensor-Based Evaluation of Liposomal Binding Behavior
Use of Liposomes to Study Vesicular Transport