H. Zhu – High-Throughput Screening Assays in Toxicology (2016)

Автор: H. Zhu
Название книги: High-Throughput Screening Assays in Toxicology
Формат: PDF
Жанр: Химия
Страницы: 178
Качество: Изначально компьютерное, E-book

Includes cutting-edge methods and protocols for HTS assays to examine toxicity
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.
Topics
Pharmacology / Toxicology
Contents
In Vitro Toxicological High – Thoughput Screening Methods
Monitoring Ligand-Activated Protein–Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay
Mitochondrial Membrane Potential Assay
Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway
Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators
Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity
Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway
Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells
Determination of Histone H2AX Phosphorylation in DT40 Cells
High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells
In Vivo Toxicological High – Thoughput Screening Methods
Better, Faster, Cheaper: Getting the Most Out of High- Throughput Screening with Zebrafish
Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans
In Silico High – Thoughput Screening Toxcity Data Analysis
1A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling
Correction of Microplate Data from High-Throughput Screening
CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints
Accounting Artifacts in High-Throughput Toxicity Assays
Accessing the High-Throughput Screening Data Landscape
Curating and Preparing High-Throughput Screening Data for Quantitative Structure-Activity Relationship Modeling