S. Nagar – Enzyme Kinetics in Drug Metabolism (2014)

Автор: S. Nagar
Название книги: Enzyme Kinetics in Drug Metabolism
Формат: PDF
Жанр: Химия
Страницы: 541
Качество: Изначально компьютерное, E-book

Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning.
Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.
Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications
Fundamentals of Enzyme Kinetics
Different Enzyme Kinetic Models
Reversible Mechanisms of Enzyme Inhibition and Resulting Clinical Significance
Irreversible Enzyme Inhibition Kinetics and Drug–Drug Interactions
Multienzyme Kinetics and Sequential Metabolism
Consideration of the Unbound Drug Concentration in Enzyme Kinetics
Enzyme Kinetics of Oxidative Metabolism: Cytochromes P450
Enzyme Kinetics, Inhibition, and Regioselectivity of Aldehyde Oxidase
Enzyme Kinetics of Conjugating Enzymes: PAPS Sulfotransferase
Enzyme Kinetics of Uridine Diphosphate Glucuronosyltransferases (UGTs)
Principles and Experimental Considerations for In Vitro Transporter Interaction Assays
Rationalizing Underprediction of Drug Clearance from Enzyme and Transporter Kinetic Data: From In Vitro Tools to Mechanistic Modeling
A Structural Model for the Mass Action Kinetic Analysis of P-gp Mediated Transport Through Confluent Cell Monolayers
Systems Biology Approaches to Enzyme Kinetics: Analyzing Network Models of Drug Metabolism
Variability in Human In Vitro Enzyme Kinetics
Sources of Interindividual Variability
Case Study 1. Practical Considerations with Experimental Design and Interpretation
Case Study 2. Practical Analytical Considerations for Conducting In Vitro Enzyme Kinetic Studies
Case Study 3. Application of Basic Enzyme Kinetics to Metabolism Studies: Real-Life Examples