A. Kianianmomeni – Optogenetics (2016)

Автор: A. Kianianmomeni
Название книги: Optogenetics
Формат: PDF
Жанр: Биологические науки
Страницы: 423
Качество: Изначально компьютерное, E-book

This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications.
Optogenetics: Basic Concepts and Their Development
Natural Resources for Optogenetic Tools
Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Reversible Photoregulation of Gene Expression and Translation
Controlling Protein Activity and Degradation Using Blue Light
Photo Control of Protein Function Using Photoactive Yellow Protein
A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Optogenetic Control of Pancreatic Islets
Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Enhancing Channelrhodopsins: An Overview
Optogenetics in Drosophila Neuroscience
Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats
Combined Optogenetic and Chemogenetic Control of Neurons
Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
Optogenetic Approaches for Mesoscopic Brain Mapping
Optogenetic Tools for Confined Stimulation in Deep Brain Structures
Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic Hydrogels
Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias