A. Lorence – Recombinant Gene Expression (2012)
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Автор: A. Lorence
Название книги: Recombinant Gene Expression
Формат: PDF
Жанр: Биологические науки
Страницы: 642
Качество: Изначально компьютерное, E-book
This third edition of Recombinant Gene Expression is a lot more than just an update of the previous edition. Although some of the authors that contributed in 2004 were also invited to participate in this new project, this volume contains brand new protocols and topics not covered before.
While organizing a book of such an extensive topic as gene expression, it was indispensable to pick and choose from the multitude of strategies, vectors, promoters, and so on, so the coverage of topics is far from exhaustive. Some expression systems were omitted because of size limitations and even within areas presented unavoidably; some research approaches were unevenly treated.
The information provided in Recombinant Gene Expression , is organized in sections by biological host: Bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells, presenting one or two authoritative reviews and several protocol chapters in each section. Each chapter concludes with a section containing excellent notes where authors offer their valuable expertise of scientists and their personal views of strategy planning, as well as a variety of approaches, and alternatives that will surely be useful and inspiring to you, the reader.
Content
General Aspects
Prokaryotes
Lower Eukaryotes
Fungi
Plants and Plant Cells
Animals and Animal Cells
Описание
A. Lorence – Recombinant Gene Expression (2012) — практическое руководство по методам рекомбинантной экспрессии генов в различных биологических системах. Книга подробно разбирает современные подходы к клонированию, выбору векторов, оптимизации экспрессии и очистке рекомбинантных белков.
Авторы рассматривают как классические, так и новейшие технологии: экспрессию в бактериях, дрожжах, насекомых, млекопитающих и растительных клетках. Особое внимание уделено проблемам растворимости, посттрансляционных модификаций, масштабированию процессов и анализу полученных продуктов.
- Молекулярным биологам и биотехнологам, занимающимся производством рекомбинантных белков
- Научным сотрудникам и аспирантам лабораторий, работающих с генной инженерией
- Специалистам биотехнологических компаний, которым нужны рабочие протоколы
- Студентам старших курсов и магистратуры биологических специальностей
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