D. Walls – Protein Chromatography (2017)

Автор: D. Walls
Название книги: Protein Chromatography
Формат: PDF
Жанр: Биологические науки
Страницы: 423
Качество: Изначально компьютерное, E-book

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of contents
A Synopsis of Proteins and Their Purification
Gel-Filtration Chromatography
Immunoaffinity Chromatography: Concepts and Applications
Avoiding Proteolysis During Protein Purification
Scale-Up of Protein Purification: Downstream Processing Issues
Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources
Stability: Enhancement and Measurement
Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Storage and Lyophilization of Pure Proteins
Differential Precipitation and Solubilization of Proteins
Ion-Exchange Chromatography: Basic Principles and Application
Protein Quantitation and Analysis of Purity
Purification of Proteins Fused to Maltose-Binding Protein
Purification of Polyhistidine-Tagged Proteins
Purification of Antibodies Using Affinity Chromatography
Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
Measuring Protein–Protein Interactions Using Biacore
Hydrophobic Interaction Chromatography
Fast Protein Liquid Chromatography
Clinical Proteomics: Liquid Chromatography–Mass Spectrometry (LC–MS) Purification Systems
Strategies for the Purification of Membrane Proteins
Antimicrobial Peptide Production and Purification
Lectin Affinity Chromatography (LAC)