E. Egelman – Comprehensive Biophysics (2012)

Автор: E. Egelman
Название книги: Comprehensive Biophysics
Формат: PDF
Жанр: Биологические науки
Страницы: 3207
Качество: Изначально компьютерное, E-book

Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics will help bridge that communication gap.
Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics provides definitive introductions to a broad array of topics, uniting different areas of biophysics research – from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource – a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge.
Key Features
– Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding
– The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research
– Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource
Contents
Volume 1 Biophysical Techniques for Structural Characterization of Macromolecules
Volume Introduction
Cantor and Schimmel – 30 Years Later
Efficient Strategies for Production of Eukaryotic Proteins
X-Ray Crystallography: Crystallization
X-Ray Crystallography: Data Collection Strategies and Resources
Phasing of X-ray Data
Refinement of X-ray Crystal Structures
Structure Validation and Analysis
Introduction to Solution State NMR Spectroscopy
Solid State NMR Methods
The Hybrid Solution/Solid-State NMR Method for Membrane Protein Structure Determination
Labeling Techniques
NMR Spectroscopy: NMR Relaxation Methods
Structure Determination of Macromolecular Complexes by Cryo-Electron Microscopy in vitro and in situ
Analysis of 2-D Crystals of Membrane Proteins by Electron Microscopy
Cryo-Electron Microscopy and Tomography of Virus Particles
Mass Spectrometry
Small and Wide Angle X-ray Scattering from Biological Macromolecules and their Complexes in Solution
Ultrafast Structural Dynamics of Biological Systems
Electron Magnetic Resonance
Computation of Structure, Dynamics, and Thermodynamics of Proteins
Rapid Mixing Techniques for the Study of Enzyme Catalysis
Optical Spectroscopy
Fluorescence and FRET: Theoretical Concepts 101
Volume 2 Biophysical Techniques for Characterization of Cells
Volume Introduction
Elucidating Cellular Structures
Confocal Microscopy
Fluorescence Lifetime Microscopy: The Phasor Approach
Super-Resolution Microscopy
Studying the Macromolecular Machinery of Cells in situ by Cryo-Electron Tomography
Visualizing Sub-cellular Organization Using Soft X-ray Tomography
Atomic Force Microscopy
Super-Resolution Near-Field Optical Microscopy
CARS Microscopy
Elucidating Cellular Dynamics
Quantitative Fluorescent Speckle Microscopy
Fluorescence Correlation Spectroscopy
Image Correlation Spectroscopy
The Basics and Potential of Single-Molecule Tracking in Cellular Biophysics
Volume 3 The Folding of Proteins and Nucleic Acids
Protein Folding
Combining Simulation and Experiment to Map Protein Folding
Globular Proteins
Energetics of Protein Folding
Fast Events in Protein Folding
Intermediates in Protein Folding
Characterization of the Denatured State
Single-Molecule Spectroscopy of Protein Folding
Simulation Studies of Force-Induced Unfolding
Protein and Nucleic Acid Folding: Domain Swapping in Proteins
Intrinsically Disordered Proteins
Chaperones and Protein Folding
Protein Switches
Repeat/Non-Globular Proteins
The Folding of Repeat Proteins
Membrane Proteins
The Membrane Factor: Biophysical Studies of Alpha Helical Transmembrane Protein Folding
Nucleic Acid Folding
Effect of Protein Binding on RNA Folding
Volume 4 Molecular Motors and Motility
Introduction
General Theoretical Considerations
Microscopic Reversibility and Free-Energy Transduction by Molecular Motors and Pumps
Actin
Structure and Dynamic States of Actin Filaments
Actin Filament Nucleation and Elongation
Mechanical Properties of Actin Networks
Microtubles
Tubulin and Microtubule Structure: Mechanistic Insights into Dynamic Instability and Its Biological Relevance
Force Generation by Dynamic Microtubule Polymers
Myosin
Myosin Motors: Structural Aspects and Functionality
Myosin Motors: Kinetics of Myosin
Single Molecule Fluorescence Techniques for Myosin
Muscle
Cell-Based Studies of the Molecular Mechanism of Muscle Contraction
Spectroscopic Probes of Muscle Proteins
Thin Filament Regulation
Smooth Muscle and Myosin Regulation
Non-Muscle Motility
Intracellular Transport: Relating Single-Molecule Properties to In Vivo Function
Mechanical Forces in Mitosis
Kinesin
Kinesin Structure and Biochemistry
Kinesin Single-Molecule Mechanics
Dynein
Cytoplasmic Dynein: Its ATPase Cycle and ATPase-dependent Structural Changes
Axonemal Motility
The Ribosome
Viral DNA Packaging Motors

Volume 5 Membranes
Volume Introduction
Biophysics of Membranes
Lipid Bilayers
Lipid Bilayer Structure
Membrane Domains and Their Relevance to the Organization of Biological Membranes
Atomic Force Microscopy and Fluorescence Microscopy of Lipid Bilayers
Detergent Interactions with Lipid Bilayers and Membrane Proteins
Membrane Proteins
Atomic Force Microscopy and Electron Microscopy of Membrane Proteins
Solution NMR Spectroscopy of Integral Membrane Proteins
Structure and Folding of Outer Membrane Proteins
Pore-Forming Toxins
Interactions of Antimicrobial Peptides with Lipid Bilayers
Membrane Recruitment of Signaling Domains
Membrane Protein–Lipid Match and Mismatch
Supported Membranes – Structure and Interactions
The Biophysics of Membrane Fusion
Mechanisms of Enveloped Virus Entry by Membrane Fusion
Computer Simulation of Membrane Dynamics
Single Molecule Measurements in Membranes
Volume 6 Channels
Channel Proteins – An Introduction
Structure-Function Correlates of Glutamate-Gated Ion Channels
Gating Dynamics of the Potassium Channel Pore
Biophysics of TRP Channels
Mechanosensory Transduction
Structures and Mechanisms in Chloride Channels
Biophysics of Ceramide Channels
Voltage Gated Proton Channels
STIM1-ORAI1 Store-Operated Calcium Channels
Structure–Function Correlates in Plant Ion Channels
Volume 7 Cell Biophysics
Introduction
Biophysics of Cell-Matrix Adhesion
Biophysics of Selectin-Mediated Cell Adhesion
Biophysics of Cadherin-Mediated Cell–Cell Adhesion
Understanding How Dividing Cells Change Shape
Biophysics of Bacterial Cell Growth and Division
Biophysics of Three-Dimensional Cell Motility
Biophysics of Molecular Cell Mechanics
Biophysics of Nuclear Organization and Dynamics
Cell-Extracellular Matrix Mechanobiology in Cancer
Biomechanics of Cell Motility
Biophysics of Cell Developmental Processes: A Lasercutter's Perspective
Bacterial Organization in Space and Time
Volume 8 Bioenergetics
on Electrochemical Gradients, Roles and Measurements
Structure-Function Relationships in P-Type ATPases
Rotational Catalysis by F1-ATPase
The Rotary Bacterial Flagellar Motor
Electron Transfer Chains: Structures, Mechanisms and Energy Coupling
Light Capture in Photosynthesis
The Structure-Function Relationships of Photosynthetic Reaction Centers
Molecular Aspects of the Translocation Process by ABC Proteins
Structural and Mechanistic Aspects of Mitochondrial Transport Proteins
Light Capture and Energy Transduction in Bacterial Rhodopsins and Related Proteins
Transporters and Co-transporters in Theory and Practice
Membrane Proteins for Secondary Active Transport and their Molecular Mechanisms
Volume 9 Simulation and Modelling
Volume Introduction
Theoretical Biophysics: A Cornerstone of Understanding in Modern Biology and Biomedicine
In Silico Approaches to Structures and Function of Cell Components and Their Aggregates
Coarse-Grained Methods: Theory
In Silico Coarse-Grained Approaches to Structural Dynamics and Function of Proteins and their Assemblies
Coarse Grained Methods: Applications to Membranes
Dynamics of Very Large Systems: The Ribosome
New Technologies for Molecular Dynamics Simulations
Simulations of Molecular Mechanisms
Molecular Modeling and Simulations of Transporter Proteins – The Transmembrane Allosteric Machinery
Gene Protein Coupled Receptors
Quantum Mechanical Methods for Enzyme Modeling: Accurate Computation of Kinetic Isotope Effects
MNDO-PSDCI and the Analysis of the Photophysical Properties of Visual Chromophores and Retinal Proteins
In Silico Approaches to Structure and Function of Cell Components and Their Assemblies
Molecular Electrostatics and Solvent Effects
Interactions of the Cell Membrane with Integral Proteins
Modeling of Interaction Networks in the Cell
Theory and Mathematical Methods
Mathematical Modeling of Complex Biological Systems
From Genes and Molecules to Organs and Organisms: Heart
Biophysical Representation of Kidney Function
Systems Immunology: A Primer for Biophysicists