J. Griffiths – Analysis of Protein Post-Translational Modifications by Mass Spectrometry

Автор: J. Griffiths
Название книги: Analysis of Protein Post-Translational Modifications by Mass Spectrometry
Формат: PDF
Жанр: Биологические науки
Страницы: 401
Качество: Изначально компьютерное, E-book

Provides a comprehensive overview of the various ways mass spectrometry can be used for the detection and analysis of protein post-translational modifications
Post-translational modification of a protein adds significant diversity and function to the proteome. It may involve the addition of functional groups to amino acids, covalent linkage of other proteins or fatty acids, a change in the chemical nature of an amino acid or structural changes resulting from proteolytic cleavage or disulfide bridge formation. Over the last 10 years, mass spectrometry has become a critical tool in the analysis of proteins structure, quantity and modification. Of the 300 or so PTMs known, this book sets out to describe mass spectrometry-based methods for the analysis of some of the most well studied.
Providing a single starting reference point for this growing and important field, with contributions from renowned experts in the analysis of all of the major PTMs and containing key background, references and summarizing the current state-of-the-art, this book is an indispensable tool for students and practitioners of protein mass spectrometry.
Contents
Introduction
Identification and Analysis of Protein Phosphorylation by Mass Spectrometry
Analysis of Protein Glycosylation by Mass Spectrometry
Protein Acetylation and Methylation
Tyrosine Nitration
Mass Spectrometry Methods for the Analysis of Isopeptides Generated from Mammalian Protein Ubiquitination and SUMOylation
The Deimination of Arginine to Citrulline
Glycation of Proteins
Biological Significance and Analysis of Tyrosine Sulfation
The Application of Mass Spectrometry for the Characterization of Monoclonal Antibody-Based Therapeutics